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Source MPP versus target MPP

MPP means micrometres per pixel: the physical tissue distance represented by one image pixel.

Value Meaning CLI
Source MPP Physical resolution of the input L0 image --source-mpp in the main CLI; --slide-mpp in run.sh
Target MPP Physical resolution requested for model tiles Existing engine --mpp; default 0.5

They are not interchangeable. Target MPP tells the workflow how to resample once source scale is known; it cannot repair unknown source scale.

Use source MPP from, in order:

  1. scanner/export provenance from the imaging facility;
  2. a trusted sidecar generated by the export; or
  3. reliable embedded TIFF/OpenSlide metadata.

Generic TIFF DPI/resolution tags can describe display conventions rather than tissue scale. If the source scale cannot be established reliably, TumorQuantAI fails closed. Guessing a plausible MPP can silently change the physical field seen by the model.

The public tutorial manifest records source MPP 0.261780 for alias 022. That verified dataset-specific value is not a universal default for other slides.

Both source and target MPP are recorded in summary/summary.json and summary/run_metadata.json.

Next: read sampling and reproducibility.